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Nanoscale Rotors Constructed From DNA – Smallest Move-Pushed Motors within the World

Artist impression of the flow-driven DNA rotor. Credit score: Cees Dekker Lab / SciXel


Flow-Driven DNA Rotor

Artist impression of the flow-driven DNA rotor. Credit score: Cees Dekker Lab / SciXel

Scientists have constructed the smallest flow-driven motors on this planet. Impressed by iconic Dutch windmills and organic motor proteins, they created a self-configuring flow-driven rotor from DNA that converts vitality from {an electrical} or salt gradient into helpful mechanical work. The outcomes open new views for engineering energetic robotics on the nanoscale. The paper by Delft College of Expertise researchers might be printed at present in Nature Physics.

Elusive

Rotary motors have been the powerhouses of human societies for millennia. We are able to look again in historical past to the windmills and waterwheels throughout the Netherlands and the world. Immediately, superior offshore wind generators drive our green-energy future.

“These rotary motors, pushed by a stream, additionally characteristic prominently in organic cells. An instance is the FoF1-ATP synthase, which produces the gasoline that cells must function. However the artificial building on the nanoscale has up to now remained elusive,” says Dr. Xin Shi, postdoctoral researcher within the lab of professor Cees Dekker within the division of Bionanoscience at Delft College of Expertise (TU Delft).

“Our flow-driven motor is constituted of DNA material. This structure is docked onto a nanopore, a tiny opening, in a thin membrane. The DNA bundle of only 7 nanometer thickness self-organizes under an electric field into a rotor-like configuration, that subsequently is set into a sustained rotary motion of more than 10 revolutions per second,” says Shi, first author of the publication in the journal Nature Physics.

DNA origami

“For already 7 years, we have been trying to build such rotary nanomotors synthetically from the bottom up. We use a technique called DNA origami, in collaboration with Hendrik Dietz’s lab from the Technical University of Munich,” adds Cees Dekker, who supervised the research. This technique uses the specific interactions between complementary DNA base pairs to build 2D and 3D nano-objects. The rotors harness energy from a water and ion flow. This is established through an applied voltage or even simpler: by having different salt concentrations on the two sides of the membrane. The latter is actually one of the most abundant energy sources in biology that powers various critical processes, including cellular fuel synthesis and cell propulsion.

Solving a puzzle

This achievement is a milestone, as it is the first-ever experimental realization of flow-driven active rotors at the nanoscale. When the researchers first observed the rotations, however, they were puzzled: how could such simple DNA rods exhibit these nice, sustained rotations? The puzzle was solved in discussions with theorist Ramin Golestanian and his team at the Max Planck Institute for Dynamics and Self-Organization in Göttingen. They modeled the system and revealed the fascinating self-organization process where the bundles spontaneously deform into chiral rotors that then couple to the flow from the nanopores.

From simplicity to rational design

“This self-organization process truly shows the beauty of simplicity,” says Shi. But the importance of this work does not stop at this simple rotor itself. The technique and physical mechanism behind it establish an entirely new direction of building synthetic nanomotors: flow-driven nanoturbines, which is, a surprisingly unexplored field by scientists and engineers. “You would be surprised how little we knew and achieved on building such flow-driven nanoturbines, especially given the millennia-old knowledge we have on building their macroscale counterparts, and the critical roles they fulfill in the life itself,” says Shi.

In a further step (which is in preprint) the group has used the knowledge they learned from building this self-organized rotor to make the next important advance: the first rationally designed nanoscale turbine. “Like how science and technologies always work, we started from a simple pinwheel, now are able to recreate the beautiful Dutch windmills, but this time with a size of only 25 nm, the size of one single protein in your body,” says Shi, “and we demonstrated their ability to carry loads.”

“And now, the rotation direction was set by the designed chirality,” Dekker adds. “Left-handed turbines rotated clockwise; right-handed ones rotated anticlockwise.”

Steam engine

Next to better understanding and mimicking motor proteins such as FoF1-ATP synthase, the results open new perspectives for engineering active robotics at the nanoscale. Shi: “What we have demonstrated here is a nanoscale engine that is truly able to transduce energy and do work. You could draw an analogy with the first invention of the steam engine in the 18th century. Who could have predicted then how it fundamentally changed our societies? We might be in a similar phase now with these molecular nanomotors. The potential is unlimited, but there is still a lot of work to do.”

Reference: “Sustained unidirectional rotation of a self-organized DNA rotor on a nanopore” 4 August 2022, Nature Physics.
DOI: 10.1038/s41567-022-01683-z





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